ANTIPYRETIC, ANTICONVULSANT, AND HEPATOPROTECTIVE PROPERTIES OF AQUEOUS EXTRACT OF ATRIPLEX HALIMUS LEAVES: IN SILICO AND IN VIVO STUDIES

Abstract

The main objective of using natural healing remedies is to mitigate the adverse effects associated with synthetic pharmaceuticals. This study aimed to assess the potential of a water-based extract derived from the leaves of Atriplex halimus in alleviating fevers, preventing seizures, and safeguarding the liver in mice using in vivo models and in silico methods. The investigation includes the protection of mice's livers from damage induced by paracetamol and monitoring liver markers such as ALP, ALT, AST, and total bilirubin in the blood, as well as assessing superoxide dismutase (SOD), MDA, catalase (CAT), and GSH levels in liver tissue.
Administration of plant extracts and silymarin to rats significantly mitigated the adverse effects of paracetamol on serum and tissue markers. Histological analysis of the liver revealed a notable impact of the extracts in reducing the severity of liver damage, with hepatoprotective efficacy comparable to that of silymarin.

Significant antipyretic effects were also observed within two hours of administering various doses of the extract, and these effects persisted throughout the experiment. Furthermore, the plant extract was found to be efficient to protect from seizure 33.34% to 83.33% of the mice treated with isoniazid and diazepam.
Finally, the pharmaceutical properties, including pharmacokinetics and toxicity of the main compounds found in A. halimus extract, were examined by ADMET simulations, and molecular docking studies were employed to investigate interactions between these compounds and drug targets.